Journal: Nature

Article Title: Circulating myeloid-derived MMP8 in stress susceptibility and depression.

doi: 10.1038/s41586-023-07015-2

Figure Lengend Snippet: Fig. 3 | Stress-induced increase in MMP8 is associated with altered ECS in the NAc. a, Plasma levels of MMP8 after CSDS. One-way ANOVA with Bonferroni post hoc test (n = 8 CON, 16 SUS and 7 RES mice). b, Correlation of MMP8 in circulation with SI ratio (n = 31 CON, SUS and RES mice; two-tailed Pearson correlation coefficient). c, Serum MMP8 levels in patients with MDD (n = 40) and healthy controls (HC) (n = 29). Two-tailed Student’s t-test. d, Correlation between MMP8 and Perceived Stress Scale (n = 68 HC and MDD; two-tailed Pearson correlation coefficient). e, Normalized MMP8 protein levels in NAc lysates of CON (n = 6), SUS (n = 5) and RES (n = 6) mice. One-way ANOVA with Bonferroni post hoc test; each data point represents three pooled NAcs. f,g, Experimental outline (f) and representative immunofluorescence image of the NAc of SUS mice that were injected retro-orbitally with biotinylated rMMP8 (g). Scale bars, 10 µm. Three to four slices per brain in three mice.

Article Snippet: Enzyme-linked immunosorbent assays were performed according to the manufacturer’s instructions (mouse MMP8: Abcam, ab206982; human MMP8: R&D Systems, DMP800B).

Techniques: Clinical Proteomics, Two Tailed Test, Immunofluorescence, Injection